Chopped Meat Carbohydrate Medium with Rumen Fluid

[所属分类:培养基配方] [发布时间:2020-4-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chopped Meat Carbohydrate
Medium with Rumen Fluid

培养基配方
Composition per 1390.0mL:
Peptone ........................................................................30.0g
K2HPO4 ..........................................................................5.0g
Yeast extract....................................................................5.0g
Glucose..........................................................................4.0g
Cellobiose.......................................................................1.0g
Maltose .........................................................................1.0g
Starch...........................................................................1.0g
L- Cysteine·HCl·H2O ...........................................................0.5g
Chopped meat extract filtrate....................................................1.0L
Chopped meat extract solids...................................................200.0mL
Rumen fluid...................................................................150.0mL
Resazurin (0.025% solution) ....................................................4.0mL
pH 7.0 ± 0.2 at 25°C
Chopped Meat Extract:
Composition per liter:
Beef or horse meat..............................................................500.0g
NaOH (1N solution)..............................................................25.0mL
Preparation of Chopped Meat Extract: Use lean beef or horse
meat. Remove fat and connective tissue. Grind. Add meat and NaOH
to distilled/deionized water and bring volume to 1.0L. Gently heat and
bring to boiling while stirring. Cool to 25°C. Remove fat from surface.
Filter. Reserve ground meat particles and filtrate. Add distilled/deion-
ized water to filtrate and bring volume to 1.0L.
Preparation of Medium: To 1.0L of chopped meat extract filtrate,
add the remaining components, except  L- cysteine·HCl·H2O and
chopped meat solids. Mix thoroughly. Gently heat to boiling. Cool to
room temperature. Add the  L- cysteine·HCl·H2O. Adjust pH to 7.0. Dis-
tribute 1 part chopped meat solids (by volume) and 5 parts of liquid (by
volume) into tubes under O 2 -free 97% N2 + 3% H2 . Cap with rubber
stoppers and place tubes in a press. Autoclave for 15 min at 15 psi pres-
sure–121°C with fast exhaust.
Use:  For the cultivation of anaerobic bacteria, including Fusobacte-
rium prausnitzii, Eubacterium species, and Prevotella ruminicola.