Chopped Meat Glucose Broth (LMG Medium 68)

[所属分类:培养基配方] [发布时间:2020-4-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chopped Meat Glucose Broth
(LMG Medium 68)

培养基配方
Composition per liter:
Ground meat, fat free............................................................500.0g
Pancreatic digest of casein.......................................................30.0g
Glucose...........................................................................10.0g
K2HPO4 ............................................................................5.0g
Yeast extract......................................................................5.0g
L -Cysteine·HCl ..................................................................0.5g
Resazurin ........................................................................1.0mg
NaOH (1N solution)...............................................................25.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Use lean beef or horse meat. Remove fat
and connective tissue. Grind finely. Add ground meat and 25.0mL of
NaOH solution to distilled/deionized water and bring volume to
1025mL. Gently heat and bring to boiling. Continue boiling for 15 min
without stirring. Cool to room temperature. Remove fat from surface.
Filter and retain both meat particles and filtrate. Adjust volume of fil-
trate to 1.0L with distilled/deionized water. Add pancreatic digest of
casein, agar, K2HPO4 , yeast extract, and resazurin. Gently heat and
bring to boiling. Boil for 1–2 min. Add  L -cysteine·HCl. Mix thorough-
ly. Distribute 7.0mL into tubes that contain meat particles (1 part meat
particles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–
121°C.
Use: For the cultivation of various Clostridium spp.