Chopped Meat Medium with 1% Glucose

[所属分类:培养基配方] [发布时间:2020-4-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chopped Meat Medium with 1% Glucose

培养基配方
Composition per 1230.0mL:
Peptone........................................................................30.0g
Glucose .......................................................................10.0g
K2HPO4 .........................................................................5.0g
Yeast extract...................................................................5.0g
L- Cysteine·HCl·H2O...........................................................0.5g
Chopped meat extract filtrate...................................................1.0L
Chopped meat extract solids..................................................200.0mL
Resazurin (0.025% solution)....................................................4.0mL
pH 7.0 ± 0.2 at 25°C
Chopped Meat Extract:
Composition per liter:
Beef or horse meat............................................................500.0g
NaOH (1N solution)............................................................25.0mL
Preparation of Chopped Meat Extract: Use lean beef or horse
meat. Remove fat and connective tissue. Grind. Add meat and NaOH
to distilled/deionized water and bring volume to 1.0L. Gently heat and
bring to boiling while stirring. Cool to 25°C. Remove fat from surface.
Filter. Reserve ground meat particles and filtrate. Add distilled/deion-
ized water to filtrate and bring volume to 1.0L.
Preparation of Medium: To 1.0L of chopped meat extract filtrate,
add the remaining components, except the  L- cysteine·HCl·H2O and
chopped meat solids. Mix thoroughly. Gently heat to boiling. Cool to
room temperature. Add the  L- cysteine. Adjust pH to 7.0. Distribute 1
part chopped meat solids (by volume) and 5 parts of liquid (by volume)
into tubes under O2 -free 97% N2 + 3% H2 . Cap with rubber stoppers
and place tubes in a press. Autoclave for 15 min at 15 psi pressure–
121°C with fast exhaust.
Use:  For the cultivation and maintenance of anaerobic bacteria,
including Bacteroides disiens, Coprococcus eutastus, Eubacterium
formicigenerans, Prevotella disiens, Ruminococcus torques, and
Streptococcus hansenii.