Christensen Agar

[所属分类:培养基配方] [发布时间:2020-4-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Christensen Agar

培养基配方
Composition per liter:
Agar.............................................................................15.0g
NaCl..............................................................................5.0g
Sodium citrate....................................................................3.0g
KH2PO4 ...........................................................................1.0g
Yeast extract.....................................................................0.5g
Glucose...........................................................................0.2g
L -Cysteine·HCl·H2O.............................................................0.1g
Phenol Red......................................................................12.0mg
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Dispense into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Allow
tubes to cool in a slanted position.
Use: For the differentiation of enteric pathogens, especially members
of the Enterobacteriaceae, and coliforms based on their ability to utilize
citrate as a carbon source. Bacteria that can utilize citrate turn the
medium pink-red.