Christensen Citrate Sulfide Medium

[所属分类:培养基配方] [发布时间:2020-4-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Christensen Citrate Sulfide Medium

培养基配方
Composition per liter:
Agar .....................................................................15.0g
NaCl.......................................................................5.0g
Sodium citrate·2H2O.......................................................3.0g
KH2HPO4 ...................................................................1.0g
Yeast extract..............................................................0.5g
Ferric citrate.............................................................0.2g
Ammonium citrate...........................................................0.2g
Glucose ...................................................................0.2g
L -Cysteine·HCl·H2O .....................................................0.1g
Na2S2O3 ·5H2O.............................................................0.08g
Phenol Red................................................................0.012g
pH 6.7± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Dispense into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Allow
tubes to cool in a slanted position.
Use: For the differentiation of enteric pathogens, especially members of
the Enterobacteriaceae, and coliforms based on their ability to utilize cit-
rate as a carbon source and production of H2S. Bacteria that can utilize
citrate turn the medium pink-red. H2S production appears as a blacken-
ing of the butt of the tube.