CHROMagar™ Orientation

[所属分类:培养基配方] [发布时间:2020-4-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CHROMagar™ Orientation

培养基配方
Composition per liter:
Peptone....................................................................16.0g
Meat extract ..............................................................16.0g
Yeast extract..............................................................16.0g
Agar ......................................................................15.0g
Chromogenic mix ............................................................2.0g
pH 7.0 ± 0.2 at 25°C
Source:  CHROMagar Orientation is available from CHROMagar Mi-
crobiology. Prepared medium is also available from BD Diagnostic
Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat in a boil-
ing water bath or steam bath. Shake periodically during heating to dis-
solve components. Heat long enough with shaking every 5 min to
ensure complete dissolution. Do not overheat. Cool to 45–50°C. Pour
into sterile Petri dishes.
Use: For the differentiation and presumptive identification of Gram-neg-
ative bacteria and Enterococcus spp. For use in identifying urinary tract
pathogens. Isolates produce characteristic diagnostic colors, e.g., Escheri-
chia coli produces pinto red colonies.