Chromatium Medium (ATCC Medium 1449)

[所属分类:培养基配方] [发布时间:2020-4-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chromatium Medium
(ATCC Medium 1449)

培养基配方
Composition per liter:
KH2PO4 ...................................................................0.5g
NH4Cl ....................................................................0.4g
MgSO4 ·7H2O..............................................................0.2g
CaCl2 ·2H2O.............................................................0.05g
Disodium EDTA ...........................................................0.01g
Trace elements...........................................................1.0mL
NaHCO3 solution.........................................................50.0mL
Na2S·9H2O solution.....................................................50.0mL
Sodium pyruvate solution................................................50.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements:
Composition per liter:
Disodium EDTA ...........................................................5.2g
FeCl2 ·4H2O.............................................................1.5g
CoCl2 ·6H2O ...........................................................0.19g
Na2MoO4 ·2H2O ........................................................0.188g
MnCl2 ·4H2O.............................................................0.1g
ZnCl2 ..................................................................0.07g
VOSO4 ·2H2O............................................................0.03g
NiCl2 ·6H2O...........................................................0.025g
H3BO3 ..................................................................6.0mg
CuCl2 ·2H2O............................................................2.0mg
Na2SeO3 ................................................................2.0mg
Preparation of Trace Elements: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
NaHCO3 Solution:
Composition per 50.0mL:
NaHCO3 ..................................................................2.0g
Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deion-
ized water and bring volume to 50.0mL. Filter sterilize. Use freshly pre-
pared solution.
Na2S·9H2O Solution:
Composition per 50.0mL:
Na2S·9H2O...............................................................1.0g
Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 50.0mL. Autoclave for 15
min at 15 psi pressure–121°C. Use freshly prepared solution.
Sodium Pyruvate Solution:
Composition per 50.0mL:
Sodium pyruvate..........................................................0.5g
Preparation of Sodium Pyruvate Solution: Add NaHCO3 to
distilled/deionized water and bring volume to 50.0mL. Filter sterilize.
Use freshly prepared solution. Sodium acetate may be substituted for
the sodium pyruvate.
Preparation of Medium: Add components, except NaHCO3 solu-
tion, Na2S·9H2O solution, and sodium pyruvate solution, to distilled
deionized water and bring volume to 850.0mL. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to room temperature. Add the sterile
NaHCO3 solution, the sterile Na2S·9H2O solution, and the sterile sodi-
um pyruvate solution, in that order. Adjust the pH to 7.0. Distribute into
screw-capped tubes or flasks. Fill to capacity.
Use:  For the cultivation and maintenance of Chromatium species.