Clostridium cellobioparum Agar

[所属分类:培养基配方] [发布时间:2020-5-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium cellobioparum Agar

培养基配方
Composition per 1025.0mL:
Ground meat, fat free......................................................500.0g
Pancreatic digest of casein.................................................30.0g
Agar........................................................................15.0g
K2HPO4 ......................................................................5.0g
Yeast extract................................................................5.0g
Glucose......................................................................4.0g
Cellobiose ..................................................................1.0g
Maltose......................................................................1.0g
Soluble starch...............................................................1.0g
L -Cysteine·HCl·H2O .......................................................0.5g
Resazurin ..................................................................1.0mg
NaOH (1N solution).........................................................25.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Remove fat and connective tissue from
lean beef or horse meat. Grind meat finely. Add ground meat to
25.0mL of 1N NaOH. Add 1.0L of distilled/deionized water. Gently
heat and bring to boiling. Continue boiling for 15 min while stirring.
Cool to room temperature. Skim fat off surface. Filter suspension and
retain the filtrate and the meat particles. Bring volume of filtrate to
1.0L with distilled/deionized water. Add remaining components, ex-
cept  L -cysteine·HCl·H2O. Mix thoroughly. Gently heat and bring to
boiling. Cool to 50°–55°C. Add  L -cysteine·HCl·H2O. Adjust pH to 7.0.
Distribute 7.0mL of agar into tubes containing meat particles (use 1
part meat particles to 4–5 parts fluid). Autoclave for 15 min at 15 psi
pressure–121°C.
Use: For the cultivation of Clostridium cellobioparum.