Clostridium Selective Agar (Clostrisel Agar)

[所属分类:培养基配方] [发布时间:2020-5-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium Selective Agar
(Clostrisel Agar)

培养基配方
Composition per liter:
Pancreatic digest of casein..............................................17.0g
Agar.....................................................................14.0g
Glucose...................................................................6.0g
Papaic digest of soybean meal.............................................3.0g
NaCl......................................................................2.5g
Sodium thioglycolate......................................................1.8g
Sodium formaldehyde sulfoxylate...........................................1.0g
L- Cystine...............................................................0.25g
NaN3 ....................................................................0.15g
Neomycin sulfate.........................................................0.15g
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–118°C. Pour into sterile Petri dishes or
leave in tubes.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Use: For the selective isolation of pathogenic Clostridium species
from specimens containing mixed flora, e.g., from wounds, fecal spec-
imens, soil, and other specimens.