Clostridium sticklandii Medium

[所属分类:培养基配方] [发布时间:2020-5-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium sticklandii Medium

培养基配方
Composition per liter:
Yeast extract................................................................5.0g
L -Arginine·HCl ............................................................2.0g
L -Lysine·HCl...............................................................2.0g
NH4Cl .......................................................................2.0g
Sodium formate...............................................................2.0g
K2HPO4 .....................................................................1.75g
MgSO4 ·7H2O.................................................................0.2g
CaCl2 ·2H2O...............................................................10.0mg
FeSO4 ·7H2O...............................................................10.0mg
Na2S·H2O solution.........................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O...................................................................0.3g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except Na2S·H2O solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C. Aseptically add 0.1mL of sterile
Na2S·H2O solution to each 10.0mL of medium.
Use: For the cultivation of Clostridium sticklandii.