Clostridium thermoaceticum Medium (TYE-CO)

[所属分类:培养基配方] [发布时间:2020-5-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium thermoaceticum Medium
(TYE-CO)

培养基配方
Composition per liter:
Pancreatic digest of casein....................................................10.0g
Yeast extract...................................................................3.0g
Na2HPO4 ·12H2O.................................................................2.8g
FeSO4 ·7H2O....................................................................1.0g
NH4Cl ..........................................................................1.0g
KH2PO4 .........................................................................0.3g
MgCl2 ·6H2O ...................................................................0.2g
Resazurin .....................................................................1.0mg
Trace elements solution.......................................................10.0mL
Na2S·9H2O solution...........................................................10.0mL
Vitamin solution...............................................................5.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO4 ·7H2O...................................................................3.0g
Nitrilotriacetic acid..........................................................1.5g
CaCl2 ·2H2O...................................................................1.0g
NaCl...........................................................................1.0g
MnSO4 ·2H2O...................................................................0.5g
CoSO4 ·7H2O..................................................................0.18g
ZnSO4 ·7H2O..................................................................0.18g
FeSO4 ·7H2O...................................................................0.1g
NiCl2 ·6H2O.................................................................0.025g
KAI(SO4)2 ·12H2O.............................................................0.02g
CuSO4 ·5H2O..................................................................0.01g
H3BO3 ........................................................................0.01g
Na2 MoO4 ·2H2O...............................................................0.01g
Na2 SeO3 ·5H2O..............................................................0.3 mg
Preparation of Trace Elements Solution : Add nitrilotriacetic
acid to approximately 500.0mL distilled/deionized water. Dissolve by
adding KOH and adjust pH to 6.5. Add remaining components. Bring
volume to 1.0L with additional distilled/deionized water. Adjust pH to
7.0 with KOH.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl..........................................................10.0mg
Calcium  DL -pantothenate.................................................5.0mg
Lipoic acid ..............................................................5.0mg
Nicotinic acid............................................................5.0mg
p-Aminobenzoic acid.......................................................5.0mg
Riboflavin ...............................................................5.0mg
Thiamine·HCl ............................................................5.0mg
Biotin ...................................................................2.0mg
Folic acid................................................................2.0mg
Vitamin B 12 .............................................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Adjust pH to 7.0. Mix thor-
oughly.
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O.................................................................0.6g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium:  Add components, except Na2S·9H2O so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Sparge with 100% N2 for 15–20 min. Before autoclaving,
sparge with 100% CO (carbon monoxide). Autoclave for 15 min at 15
psi pressure–121°C. Aseptically and anaerobically add 10.0mL of ster-
ile Na2S·9H2O solution.
Caution: CO is toxic.
Use:  For the cultivation and maintenance of Clostridium thermoaceti-
cum.