Clostridium thermoaceticum Medium

[所属分类:培养基配方] [发布时间:2020-5-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium thermoaceticum Medium

培养基配方啊
Composition per liter:
Pancreatic digest of casein.....................................................5.0g
Yeast extract...................................................................5.0g
(NH4)2SO4 ......................................................................0.5g
MgSO4 ·7H2O....................................................................0.1g
Fe(NH4)2(SO4)2 ................................................................0.04g
Na2MoO4 ·2H2O.................................................................2.4mg
Resazurin......................................................................1.0mg
Phosphate solution...........................................................100.0mL
Glucose solution.............................................................100.0mL
NaHCO3  solution.............................................................100.0mL
L -Cysteine·HCl solution.....................................................10.0mL
Na2S·9H2O solution...........................................................10.0mL
pH 6.9 ± 0.2 at 25°C
Phosphate Solution:
Composition per 100.0mL:
K2HPO4 .........................................................................7.0g
KH2PO4 .........................................................................4.5g
Preparation of Phosphate Solution:  Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Glucose Solution:
Composition per 100.0mL:
D- Glucose.....................................................................18.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Sparge with
100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
NaHCO3 Solution:
Composition per 100.0mL:
NaHCO3 ........................................................................10.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Sparge
with 100% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C.
L -Cysteine·HCl Solution:
Composition per 10.0mL:
L -Cysteine·HCl..............................................................0.3g
Preparation of  L -Cysteine·HCl Solution: Add  L -cysteine·HCl to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O....................................................................0.3g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except phosphate solu-
tion, glucose solution, NaHCO3 solution,  L -cysteine·HCl solution, and
Na2S·9H2O solution, to distilled/deionized water and bring volume to
680.0mL. Mix thoroughly. Sparge with 100% CO2 . Autoclave for 15
min at 15 psi pressure–121°C. Aseptically and anaerobically add
100.0mL of sterile phosphate solution, 100.0mL of sterile glucose so-
lution, 100.0mL of sterile NaHCO3 solution, 10.0mL of sterile  L -
cysteine·HCl solution, and 10.0mL of sterile Na2S·9H2O solution. Mix
thoroughly. Check that final pH is 6.9.
Use: For the cultivation and maintenance of Clostridium thermoaceti-
cum.