Clostridium thermocellum Medium (LMG Medium 42)

[所属分类:培养基配方] [发布时间:2020-5-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium thermocellum Medium
(LMG Medium 42)

培养基配方
Composition per liter:
Agar ......................................................................30.0g
Sodium-beta-glycerophosphate ...............................................6.0g
K2HPO4 .....................................................................5.5g
Yeast extract...............................................................4.5g
MgCl2 ·6H2O................................................................2.6g
KH2PO4 ....................................................................1.43g
(NH4)2SO4 ..................................................................1.3g
CaCl2 ·2H2O...............................................................0.13g
Glutathione................................................................0.25g
FeSO4 ·7H2O...............................................................1.1mg
Resazurin .................................................................1.0mg
Cellobiose solution ......................................................50.0mL
pH 7.1 ± 0.2 at 25°C
Cellobiose Solution:
Composition per 100.0mL:
Cellobiose .................................................................10.0g
Preparation of Cellobiose Solution: Add cellobiose to 100.0mL
of distilled/deionized water. Mix thoroughly. Sparge with  95% N2 + 5%
CO2 gas . Filter sterilize.
Preparation of Medium: Add components, except cellobiose solu-
tion, to 950.0mL distilled/deionized water  under 95% N2 + 5% CO2
gas atmosphere . Mix thoroughly and sparge with  95% N2 + 5% CO2
gas . Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add 50.0mL sterile cellobiose solution.
Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Clostridium thermocel-
lum.