Clostridium thermohydrosulfuricum Medium

[所属分类:培养基配方] [发布时间:2020-5-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium thermohydrosulfuricum Medium

培养基配方
Composition per liter:
Sucrose....................................................................10.0g
Pancreatic digest of casein................................................10.0g
Yeast extract...............................................................2.0g
FeSO4 ·7H2O................................................................0.2g
Na2SO3 .....................................................................0.2g
Na2S2O3 ·5H2O............................................................ 0.08g
Resazurin .................................................................1.0mg
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2
for 15 min. Autoclave for 30 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Clostridium thermohy-
drosulfuricum, Clostridium thermosaccharolyticum, Thermoanaer-
obacter ethanolicus, and Thermoanaerobacter thermohydrosulfuricus.