CM3 Medium

[所属分类:培养基配方] [发布时间:2020-5-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CM3 Medium

培养基配方
Composition per liter:
3–(N–Morpholino)propanesulfonic
acid (MOPS) buffer....................................................20.0g
Cellobiose (or Cellulose MN 300)......................................10.0g
K2HPO4 ................................................................4.4g
Urea...................................................................1.5g
L -Cysteine·HCl·H2O..................................................1.0g
MgSO4 ·7H2O...........................................................0.5g
(NH4)2SO4 .............................................................0.4g
CaCl2 ·2H2O .........................................................0.05g
FeSO4 ·7H2O..........................................................1.0mg
pH 7.1 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Clostridium species.