CMA with Lupine

[所属分类:培养基配方] [发布时间:2020-5-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CMA with Lupine

培养基配方
Composition per liter:
Agar.........................................................................20.0g
Cornmeal polenta.............................................................15.0g
Lupine stems...............................................................variable
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add cornmeal polenta to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and
bring to boiling. Continue boiling for 30 min. Filter through Whatman
#1 filter paper. Add agar to filtrate. Gently heat and bring to boiling.
Distribute 6.0mL volumes into tubes. Cut lupine stems into 8.0cm-long
pieces. Add 2–3 lupine stems per tube. Autoclave for 15 min at 15 psi
pressure–121°C. Allow tubes to cool in a slanted position.
Use:  For the cultivation of Glomerella cingulata.