CML Medium (Cooked Meat Liver Medium)

[所属分类:培养基配方] [发布时间:2020-5-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CML Medium
(Cooked Meat Liver Medium)

培养基配方
Composition per liter:
Cooked meat................................................................57.0g
Glucose....................................................................10.0g
Tryptose...................................................................10.0g
Liver infusion broth......................................................10.0mL
pH 6.9 ± 0.2 at 25°C
Liver Infusion Broth:
Composition per liter:
Beef liver, infusion from..................................................500.0g
Proteose peptone............................................................10.0g
NaCl.........................................................................5.0g
Preparation of Liver Infusion Broth:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add cooked meat to distilled/deionized
water and bring volume to 1.0L. Chill to 4°C until liquid is clear. Filter
through cheesecloth. Add remaining components to filtrate. Distribute
into tubes or flasks. Autoclave for 10 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Fusobacterium varium.