Coagulase Mannitol HiVeg Broth Base with Plasma

[所属分类:培养基配方] [发布时间:2020-5-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Coagulase Mannitol HiVeg Broth Base with Plasma

培养基配方
Composition per liter:
D -Mannitol................................................................10.0g
Plant infusion.............................................................10.0g
Plant peptone..............................................................10.0g
NaCl........................................................................5.0g
Phenol Red................................................................0.025g
Rabbit plasma, strerile, pretested normal..........................120.0-150.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium:  Add components, except rabbit plasma,
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat while stirring until boiling. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. For
detection of coagulase activity add rabbit plasma with 0.15% EDTA to
a final concentration of 12–15%. Mix thoroughly.
Use: For the cultivation and differentiation of Staphylococcus aureus
from other Staphylococcus species based on coagulase production and
mannitol fermentation. For the simultaneous detection of coagulase
production and mannitol fermentation in the differentiation of Staphy-
lococci.