Coletsos Medium

[所属分类:培养基配方] [发布时间:2020-5-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Coletsos Medium

培养基配方
Composition per 1625mL:
Potato starch..............................................................10.0g
Gelatin.....................................................................4.0g
Asparagine.................................................................2.25g
KH2PO4 .....................................................................1.5g
Na-glutamate................................................................1.0g
Na-pyruvate ................................................................1.0g
Mg-citrate................................................................0.375g
Litmus.....................................................................0.25g
Malachite green............................................................0.25g
MgSO4 .....................................................................0.15g
Activated carbon............................................................0.1g
Oligonucleotide mixture....................................................3.0mg
Egg mixture..............................................................625.0mL
Glycerol ..................................................................7.5mL
Egg Mixture:
Composition per liter:
Whole eggs ...............................................................18–24
Preparation of Egg Mixture:  Use fresh eggs, less than 1 week old.
Scrub the shells with soap. Let stand in a soap solution for 30 min.
Rinse in running water. Soak eggs in 70% ethanol for 15 min. Break
the eggs into a sterile container. Separate egg whites from egg yolks.
Combine 8 parts egg white with 2 parts egg yolk. Homogenize by shak-
ing. Filter through four layers of sterile cheesecloth into a sterile grad-
uated cylinder. Bring volume to 1.0L distilled/deionized water.
Preparation of Medium: Add glycerol to 600.0mL of distilled/de-
ionized water. Mix thoroughly. Add remaining components, except egg
mixture. Bring volume to 1.0L. Mix thoroughly. Gently heat while stir-
ring and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add 625.0mL of egg mixture. Mix
thoroughly. Distribute into sterile screw-capped tubes. Place tubes in a
slanted position. Inspissate at 85°C (moist heat) for 45 min.
Use: For the cultivation of Mycobacterium tuberculosis.