- 定做培养基/定制培养基
- 2020年版中国药典
- 促销/特价商品
- 院感/疾控/体外诊断/采样管
- 样品采集与处理(均质)产品
- 按标准检索培养基
- 颗粒培养基
- 标准菌株生化鉴定试剂盒
- 预灌装即用型成品培养基
- 模拟灌装用培养基
- 干燥粉末培养基
- 培养基添加剂/补充剂
- 生化反应鉴定管
- 染色液等配套产品
- 对照培养基/标准品
- 实验耗材与器具
- 生化试剂/化学试剂
- 菌种鉴定服务
Coliform Medium (CM)
山东拓普生物工程有限公司
Shandong Tuopu Biol-Engineering Co.,Ltd
Coliform Medium
(CM)
培养基配方
Composition per liter:
Bile salts No.3..........................................................20.0g
Lactose..................................................................20.0g
Proteose peptone No.3....................................................10.0g
Yeast extract.............................................................6.0g
Sodium lauryl sulfate.....................................................1.0g
Sodium deoxycholate.......................................................0.1g
Bromcresol Purple solution .............................................10.0mL
pH 7.0 ± 0.2 at 25°C
Bromcresol Purple Solution:
Composition per 100.0mL:
Bromcresol Purple .......................................................0.35g
NaOH (0.1N solution).....................................................2.0mL
Preparation of Bromcresol Purple Solution: Combine Bro-
mcresol Purple and NaOH solution. Mix thoroughly. Bring volume to
100.0mL with distilled/deionized water. Filter sterilize.
Preparation of Medium: Add components, except Bromcresol
Purple solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Add 10.0mL of Bromcresol Purple solu-
tion. Mix thoroughly. Adjust pH to 7.0 with 1N HCl. Distribute into
flasks in 95.0mL volumes. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the isolation and cultivation of coliform microorganisms
from cream.