Coliform Medium, Modified (MCM)

[所属分类:培养基配方] [发布时间:2020-5-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Coliform Medium, Modified
(MCM)

培养基配方
Composition per liter:
Lactose...............................................................20.0g
Tris(hydroxymethyl)aminomethane buffer................................12.1g
Proteose peptone No.3.................................................10.0g
Yeast extract..........................................................6.0g
Bile salts No.3........................................................1.0g
Sodium lauryl sulfate..................................................1.0g
Sodium deoxycholate....................................................0.1g
Bromcresol Purple solution ..........................................10.0mL
pH 6.8 ± 0.2 at 25°C
Bromcresol Purple Solution:
Composition per 100.0mL:
Bromcresol Purple.....................................................0.35g
NaOH (0.1N solution)..................................................2.0mL
Preparation of Bromcresol Purple Solution: Combine Bro-
mcresol Purple and NaOH solution. Mix thoroughly. Bring volume to
100.0mL with distilled/deionized water. Filter sterilize.
Preparation of Medium: Add components, except Bromcresol
Purple solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Add 10.0mL of Bromcresol Purple solu-
tion. Mix thoroughly. Adjust pH to 6.8 with 1N NH4OH. Distribute into
flasks in 95.0mL volumes. Autoclave for 25 min at 15 psi pressure–
121°C.
Use: For the isolation and cultivation of coliform microorganisms
from yogurt.