CreDm1 Medium

[所属分类:培养基配方] [发布时间:2020-5-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CreDm1 Medium

培养基配方
Composition per 1002.0mL:
Solution A.............................................................980.0mL
Solution D (Vitamin solution) ..........................................10.0mL
Solution E .............................................................10.0mL
Solution B (Trace elements solution SL-10) ..............................1.0mL
Solution C (Selentite-tungstate solution)................................1.0mL
pH 6.7–6.9 at 25°C
Solution A:
Composition per 980.0mL:
KH2PO4 ...................................................................1.4g
NH4Cl ....................................................................0.5g
MgCl2 ·6H2O .............................................................0.2g
CaCl2 ·2H2O ............................................................0.15g
Yeast extract...........................................................50.0mg
Preparation of Solution A : Add components to distilled/deionized
water and bring volume to 980.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Solution B (Trace Elements Solution SL-10):
Composition per liter:
FeCl2 ·4H2O..........................................................1.5g
CoCl2 ·6H2O.......................................................190.0mg
MnCl2 ·4H2O ......................................................100.0mg
ZnCl2 ..............................................................70.0mg
Na2MoO4 ·2H2O......................................................36.0mg
NiCl2 ·6H2O........................................................24.0mg
H3BO3 ..............................................................6.0mg
CuCl2 ·2H2O........................................................2.0mg
HCl (25% solution).................................................10.0mL
Preparation of Solution B (Trace Elements Solution SL-10):
Add FeCl2 ·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add dis-
tilled/deionized water and bring volume to 1.0L. Add remaining com-
ponents. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C.
Solution C (Selenite-Tungstate Solution):
Composition per liter:
NaOH.....................................................................0.5g
Na2WO4 ·2H2O...........................................................4.0mg
Na2SeO3 ·5H2O..........................................................3.0mg
Preparation of Solution C (Selenite-Tungstate Solution):
Add components to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Solution D (Vitamin Solution):
Composition per liter:
Pyridoxine·HCl........................................................10.0mg
Calcium  DL -pantothenate...............................................5.0mg
Lipoic acid ............................................................5.0mg
Nicotinic acid..........................................................5.0mg
p-Aminobenzoic acid.....................................................5.0mg
Riboflavin .............................................................5.0mg
Thiamine·HCl ..........................................................5.0mg
Biotin .................................................................2.0mg
Folic acid..............................................................2.0mg
Vitamin B 12 ...........................................................0.1mg
Preparation of Solution D (Vitamin Solution):  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Solution E:
Composition per 10.0mL:
Disodium- DL -malate.....................................................1.6g
Preparation of Solution E:  Add disodium- DL -malate to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Aseptically combine 980.0mL of sterile
solution A with 1.0mL of sterile solution B, 1.0mL of sterile solution
C, 10.0mL of sterile solution D, and 10.0mL of sterile solution E, in
that order. Mix thoroughly. Adjust pH to 6.7–6.9. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation of Campylobacter species.