​Desulfovibrio sulfodismutans Medium (DSMZ Medium 386)

[所属分类:培养基配方] [发布时间:2020-6-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfovibrio sulfodismutans Medium
(DSMZ Medium 386)

培养基配方
Composition per 1002.0mL:
Solution A...............................................................920.0mL
Solution C................................................................50.0mL
Solution D................................................................10.0mL
Solution F................................................................10.0mL
Solution G................................................................10.0mL
Solution B.................................................................1.0mL

Solution E ................................................................1.0mL
pH 7.1–7.4 at 25°C
Solution A:
Composition per 920.0mL:
NaCl........................................................................1.0g
KCl.........................................................................0.5g
MgCl2 ·6H2O................................................................0.4g
KH2PO4 .....................................................................0.2g
NH4Cl ......................................................................0.3g
CaCl2 ·2H2O...............................................................0.15g
Preparation of Solution A : Add components to distilled/deionized
water and bring volume to 920.0mL. Mix thoroughly. Sparge with 80%
N2  + 20% CO2 gas until saturated. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 25°C.
Solution B:
Composition per liter:
FeCl2 ·4H2O................................................................1.5g
H3BO3 ...................................................................300.0mg
CoCl2 ·6H2O ............................................................190.0mg
MnCl2 ·4H2O.............................................................100.0mg
ZnCl2 ....................................................................70.0mg
Na2MoO4 ·2H2O ...........................................................36.0mg
NiCl2 ·6H2O..............................................................24.0mg
CuCl2 ·2H2O ..............................................................2.0mg
HCl (25% solution).........................................................7.7mL
Preparation of Solution B: Add FeCl2 ·4H2O to 10.0mL of HCl so-
lution. Mix thoroughly. Add distilled/deionized water and bring vol-
ume to 1.0L. Add remaining components. Mix thoroughly. Sparge with
100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Solution C:
Composition per 100.0mL:
NaHCO3 ......................................................................5.0g
Preparation of Solution C : Add NaHCO3 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Sparge with 80%
N2  + 20% CO2  gas until saturated, approximately 20 min. Filter steril-
ize under 100% CO2 into a sterile, gas-tight 100.0mL screw-capped
bottle.
Solution D:
Composition per 10.0mL:
Na-acetate·3H2O.............................................................0.3g
Preparation of Solution D:  Add Na-acetate·3H2O to distilled/de-
ionized water and bring volume to 10.0mL. Sparge with N2 . Filter ster-
ilize. Store anaerobically.
Solution E:
Composition per 1.0mL:
Ca-D-pantothenate..........................................................50.0μg
D (+)-Biotin...............................................................10.0μg
Preparation of Solution E:  Add components to distilled/deionized
water and bring volume to 1.0mL. Mix thoroughly. Sparge with 100%
N2 . Filter sterilize.
Solution F:
Composition per 10.0mL:
Na2S·9H2O..................................................................0.4g
Preparation of Solution F:  Add Na2S·9H2O to distilled/deionized
water and bring volume to 10.0mL. Sparge with N2 . Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C. Store anaerobically.
Solution G:
Composition per liter:
NaOH.........................................................................0.5g
Na2SeO3 ·5H2O..............................................................3.0mg
Preparation of Solution G: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Sparge with 100%
N2 . Filter sterilize.
Preparation of Medium:  Add solution B, solution C, solution D,
solution E, solution F, and solution G to solution A in that order under
80% N2  + 20% CO2  gas. Adjust the pH to 7.1–7.4. When growth has
started feed culture again with same amount of solution G. After a fur-
ther 2 days repeat feeding once more.
Use: For the cultivation of Desulfovibrio sulfodismutans.