Desulfuromonas acetoxidans Medium

[所属分类:培养基配方] [发布时间:2020-6-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfuromonas acetoxidans Medium

培养基配方
Composition per 1001.0mL:
Fumaric acid ...............................................................1.5g
KH2PO4 .....................................................................1.0g
NH4Cl ......................................................................0.5g
Sodium acetate..............................................................0.5g
MgSO4 ·7H2O.................................................................0.4g
CaCl2 ·2H2O.................................................................0.1g
Resazurin ..................................................................0.5mg
NaHCO3  solution ...........................................................40.0mL
Trace elements solution SL-4 ...............................................10.0mL
Na2S·9H2O solution..........................................................6.0mL
Vitamin solution.............................................................5.0mL
pH 7.5 ± 0.2 at 25°C
NaHCO3 Solution:
Composition per 40.0mL:
NaHCO3 ......................................................................2.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 40.0mL. Mix thoroughly. Gas under
80% N2 + 20% CO2 . Autoclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution SL-4:
Composition per liter:
EDTA..........................................................................0.5g
FeSO4 ·7H2O..................................................................0.2g
Trace elements solution SL-6 ..............................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
MnCl2 ·4H2O.................................................................0.5g
H3BO3 .......................................................................0.3g
CoCl2 ·6H2O ................................................................0.2g
ZnSO4 ·7H2O.................................................................0.1g
Na2MoO4 ·2H2O .............................................................0.03g

NiCl2 ·6H2O................................................................0.02g
CuCl2 ·2H2O ...............................................................0.01g
Preparation of Trace Elements Solution SL-6: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gas under 100% N2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Preparation of Trace Elements Solution SL-4:  Add compo-
nents to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly.
Na2S·9H2O Solution:
Composition per 6.0mL:
Na2S·9H2O................................................................0.3g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Gas under 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl............................................................62.5g
Nicotinic acid............................................................25.0mg
p-Aminobenzoic acid.......................................................12.5mg
Thiamine·HCl ............................................................12.5mg
Calcium  DL -pantothenate..................................................6.5mg
Biotin.....................................................................2.5mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH
to 7.5. Gas under 100% N2 . Filter sterilize.
Preparation of Medium: Add components, except NaHCO3  solu-
tion, Na2S·9H2O solution, and vitamin solution, to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.0.
Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Aseptically and anaerobically add 40.0mL of sterile NaHCO3  solution,
6.0mL of sterile Na2S·9H2O solution, and 5.0mL of sterile vitamin so-
lution. Mix thoroughly. Aseptically and anaerobically distribute into
sterile screw-capped bottles or tubes. Fill completely, leaving only a
small gas bubble.
Use:  For the cultivation and maintenance of Desulfuromonas acetox-
idans.