Dethiosulfovibrio II Medium (DSMZ Medium 906)

[所属分类:培养基配方] [发布时间:2020-6-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dethiosulfovibrio II Medium
(DSMZ Medium 906)

培养基配方
Composition per liter:
NaCl........................................................................20.0g
Yeast extract................................................................5.0g
MgCl2 ·6H2O ................................................................3.0g
Na3 -citrate·5H2O...........................................................3.0g
Peptone .....................................................................2.0g
KH2PO4 ......................................................................1.0g
Resazurin ..................................................................0.5mg
Calcium chloride solution..................................................10.0mL
Na2S·9H2O solution........................................................10.0mL
Na2S2O3  solution..........................................................10.0mL
Trace elements solution SL-10..............................................1.0mL
pH 6.7–6.8 at 25°C
Na2S2O3 Solution :
Composition per 10.0mL:
Na2S2O3 ·5H2O............................................................2.5g
Preparation of Na2S2O3 Solution : Add Na2S2O3 ·5H2O to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Auto-
clave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to
room temperature.
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O..............................................................0.5g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution SL-10:
Composition per liter:
FeCl2 ·4H2O...........................................................1.5g
CoCl2 ·6H2O .......................................................190.0mg
MnCl2 ·4H2O........................................................100.0mg
ZnCl2 ...............................................................70.0mg
Na2MoO4 ·2H2O ......................................................36.0mg
NiCl2 ·6H2O.........................................................24.0mg
H3BO3 ................................................................6.0mg
CuCl2 ·2H2O .........................................................2.0mg
HCl (25% solution)...................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl2 ·4H2O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water
and bring volume to 1.0L. Add remaining components. Mix thoroughly.
Sparge with 80% N2 + 20% CO2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Calcium Chloride Solution:
Composition per 10.0mL:
CaCl2 ·2H2O............................................................0.2g
Preparation of Calcium Chloride Solution:  Add CaCl2 ·2H2O
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Preparation of Medium: Add components, except calcium chloride
solution, trace elements solution SL-10, Na2S·9H2O solution, and
Na2S2O3 solution, to distilled/deionized water and bring volume to
969.0mL. Mix thoroughly. Sparge with 100% N2 for 30 min. Distribute
under 100% N2 into anaerobe tubes or bottles. Autoclave for 15 min at
15 psi pressure–121°C. Aseptically and anaerobically per 1.0L of medi-
um add 10.0mL calcium chloride solution, 1.0mL trace elements solu-
tion SL-10, 10.0mL Na2S·9H2O solution, and 10.0mL Na2S2O3
solution. Mix thoroughly. The final pH should be 6.7–6.8.
Use:  For the cultivation of Dethiosulfovibrio spp.