Dey-Engley Neutralizing HiVeg Agar (D-E HiVeg Agar Disinfectant Testing)

[所属分类:培养基配方] [发布时间:2020-6-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dey-Engley Neutralizing HiVeg Agar
(D-E HiVeg Agar Disinfectant Testing)

培养基配方
Composition per liter:
Agar .......................................................................15.0g
Glucose ....................................................................10.0g
Lecithin.....................................................................7.0g
Na2S2O3 .....................................................................6.0g
Plant hydrolysate............................................................5.0g
Polysorbate 80...............................................................5.0g
NaHSO3 ......................................................................2.5g
Yeast extract................................................................2.5g
Na-thioglycollate ...........................................................1.0g
Bromcresol Purple ..........................................................0.02g
pH 7.6 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into flasks in 9.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C.
Use: For the neutralization and testing of antiseptics and disinfectants.
Dey-Engley Neutralizing HiVeg Broth
Composition per liter:
Glucose .....................................................................10.0g
Lecithin......................................................................7.0g
Na2S2O3 ......................................................................6.0g
Plant hydrolysate.............................................................5.0g
Polysorbate 80................................................................5.0g
NaHSO3 .......................................................................2.5g
Yeast extract.................................................................2.5g
Na-thioglycollate ............................................................1.0g
Bromcresol Purple............................................................0.02g
pH 7.6± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
in 9.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the neutralization and testing of antiseptics and disinfectants.