Diamonds Medium, Modified

[所属分类:培养基配方] [发布时间:2020-6-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Diamonds Medium, Modified

培养基配方
Composition per liter:
Pancreatic digest of casein..............................................20.0g
Yeast extract.............................................................1.0g
L- Cysteine·HCl·H2O.....................................................0.5g
Maltose...................................................................0.5g
L- Ascorbic acid ........................................................0.02g
Horse serum, inactivated...............................................100.0mL
Antibiotic inhibitor ...................................................10.0mL
pH 6.5 ± 0.2 at 25°C
Antibiotic Inhibitor:
Composition per 10.0mL:
Streptomycin sulfate ....................................................0.15g
Amphotericin B...........................................................0.2mg
Penicillin G..........................................................100,000U
Preparation of Antibiotic Inhibitor: Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except antibiotic inhib-
itor and horse serum, to distilled/deionized water and bring volume to
890.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add
sterile antibiotic inhibitor and horse serum. Mix thoroughly. Aseptical-
ly distribute into sterile tubes in 5.0mL volumes.
Use: For the cultivation of Trichomonas species.