Dictyoglomus Medium

[所属分类:培养基配方] [发布时间:2020-6-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dictyoglomus Medium

培养基配方
Composition per liter:
Soluble starch..................................................................5.0g
Na2HPO4 ·12H2O.................................................................4.2g
Polypeptone™....................................................................2.0g
Yeast extract...................................................................2.0g
KH2PO4 .........................................................................1.5g
L -Cysteine·HCl·H2O ..........................................................1.0g
Na2CO3 .........................................................................1.0g
NH4Cl ..........................................................................0.5g
MgCl2 ·6H2O...................................................................0.38g
CaCl2 .........................................................................0.05g
Fe(NH4)2(SO4）2 ·6H2O .......................................................0.039g
Resazurin .....................................................................2.0mg
Trace metals..................................................................10.0mL
Wolfe’s vitamin solution.....................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Trace Metals:
Composition per liter:
CoCl2 ·6H2O .................................................................0.29g
ZnSO4 ·7H2O..................................................................0.28g
Na2MoO4 ·2H2O ...............................................................0.24g
MnCl2 ·4H2O...................................................................0.2g
Na2SeO3 .....................................................................0.017g
Preparation of Trace Metals: Add components to distilled/deion-
ized water and bring volume to 1.0L. Adjust pH to 6.0 with KOH. Mix
thoroughly.
Wolfe’s Vitamin Solution:
Composition per liter:
Pyridoxine·HCl...............................................................0.01g
Thiamine·HCl ................................................................5.0mg
Riboflavin ...................................................................5.0mg
Nicotinic acid................................................................5.0mg
Calcium pantothenate .........................................................5.0mg
p-Aminobenzoic acid...........................................................5.0mg
Thioctic acid ................................................................5.0mg
Biotin .......................................................................2.0mg
Folic acid....................................................................2.0mg
Cyanocobalamin ...............................................................0.1mg
Preparation of Wolfe’s Vitamin Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Prepare and dispense medium under 100%
N2 . Add components, except Wolfe’s vitamin solution, to distilled/deion-
ized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and
bring to boiling. Continue boiling until resazurin turns colorless. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 25°C under 100% N 2 .
Aseptically add sterile Wolfe’s vitamin solution. Mix thoroughly. Adjust
pH to 7.2 if necessary. Aseptically and anaerobically distribute into sterile
tubes or flasks.
Use:  For the cultivation and maintenance of Dictyoglomus thermophi-
lum.