​Diethyl Phosphonate Agar

[所属分类:培养基配方] [发布时间:2020-6-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Diethyl Phosphonate Agar

培养基配方
Composition per liter:
Agar........................................................................12.0g
Tris(hydroxymethyl)methylamine...............................................6.0g
p-Hydroxybenzoate, Na salt .................................................0.75g
KCl..........................................................................0.2g
MgSO4 ·7H2O.................................................................0.2g
NH4Cl .......................................................................0.2g
Diethyl phosphonate solution..............................................100.0mL
pH 7.4 ± 0.2 at 25°C
Diethyl Phosphonate Solution:
Composition per 100.0mL:
Diethyl phosphonate.......................................................0.015g
Source: Diethyl phosphonate is available from Eastman Organic
Chemical Division, Rochester, NY.
Preparation of Diethyl Phosphonate Solution : Add diethyl
phosphonate to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except diethyl phospho-
nate solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 25°C. Aseptically add 100.0mL diethyl
phosphate solution. Mix. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use:  For the cultivation and maintenance of Comamonas acidovorans.