Dixon Agar

[所属分类:培养基配方] [发布时间:2020-6-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dixon Agar

培养基配方
Composition per liter:
Malt extract..................................................................30.0g
Oxbile........................................................................20.0g
Agar .........................................................................15.0g
Mycological peptone............................................................5.0g
Glycerol mono-oleate .........................................................2.50g
Tween™ 40....................................................................10.0mL
pH 5.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring until boiling. Distribute into tubes or flasks. Autoclave for 10
min at 15 psi pressure–115°C. Do not overheat or agar will not harden.
If a lower pH (3.5) is desired, cool medium to 55°C and aseptically add
100.0mL of sterile lactic acid. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the cultivation and maintenance of Malassezia species.