DNase Medium

[所属分类:培养基配方] [发布时间:2020-6-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

DNase Medium

培养基配方
Composition per liter:
Agar..........................................................................15.0g
Pancreatic digest of casein...................................................10.0g
Peptic digest of animal tissue ...............................................10.0g
L- Arabinose..................................................................10.0g
NaCl...........................................................................5.0g
Deoxyribonucleic acid..........................................................2.0g
Methyl Green..................................................................0.09g
Phenol Red....................................................................0.05g
Antibiotic solution..........................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Antibiotic Solution:
Composition per 10.0mL:
Cephalothin..................................................................0.01g
Ampicillin...................................................................5.0mg
Colistimethate...............................................................5.0mg
Amphotericin B ..............................................................2.5mg
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except antibiotic solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile com-
ponents. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the isolation and cultivation of Serratia marcescens.