Doyle and Roman Enrichment Medium

[所属分类:培养基配方] [发布时间:2020-6-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Doyle and Roman Enrichment Medium

培养基配方
Composition per liter:
Pancreatic digest of casein...............................................10.0g
Peptic digest of animal tissue............................................10.0g
NaCl.......................................................................5.0g
Sodium succinate...........................................................3.0g
Yeast extract..............................................................2.0g
Glucose ...................................................................1.0g
NaHSO3 ....................................................................0.1g
L -Cysteine·HCl·H2O .....................................................0.1g
Horse blood, lysed ......................................................70.0mL
Antibiotic solution .....................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide............................................................0.05g
Vancomycin .............................................................0.015g
Trimethoprim lactate.....................................................5.0mg
Polymyxin B...........................................................200,000U
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except antibiotic solu-
tion and horse blood, to distilled/deionized water and bring volume to
920.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile antibiotic solution and horse blood. Mix thoroughly. Asep-
tically distribute into sterile flasks in 90.0–100.0mL volumes.
Use: For the cultivation and enrichment of Campylobacter species
from foods.