DTC Agar

[所属分类:培养基配方] [发布时间:2020-6-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

DTC Agar

培养基配方
Composition per liter:
Agar.......................................................................20.0g
Pancreatic digest of casein................................................15.0g
NaCl........................................................................5.0g
Papaic digest of soybean meal...............................................5.0g
Deoxyribonucleic acid.......................................................2.0g
Toluidine Blue O............................................................0.1g
Cephalothin solution......................................................10.0mL
Cephalothin Solution:
Composition per 10.0mL:
Cephalothin................................................................1.0g
Preparation of Cephalothin Solution: Add cephalothin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except cephalothin so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile ce-
phalothin solution. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the isolation and cultivation of Serratia species. Serratia
appear as colonies with red halos.