A 3 Agar

[所属分类:培养基配方] [发布时间:2019-9-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

A 3 Agar

Composition per 202.4mL:
Agar base ...............................................................................140.0mL
Supplement solution ................................................................62.4mL
pH 6.0 ± 0.2 at 25°C
Agar Base:
Composition per liter:
Pancreatic digest of casein..........................................................17.0g
Ionagar No. 2 ................................................................................7.5g
NaCl..............................................................................................5.0g
Papaic digest of soybean meal......................................................3.0g
K 2 HPO 4 .........................................................................................2.5g
Glucose .........................................................................................2.5g
Source:  Ionagar No. 2 is available from Oxoid Unipath.
Preparation of Agar Base:  Add components, except agar, to dis-
tilled/deionized water and bring volume to 1.0L. Adjust pH to 5.5. Add
agar. Mix thoroughly. Gently heat and bring to boiling. Distribute into
screw-capped bottles in 140.0mL volumes. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C.
Supplement Solution:
Composition per 62.4mL:
Horse serum-urea solution.......................................................40.0mL
Fresh yeast extract solution......................................................20.0mL
Penicillin solution......................................................................2.0mL
Phenol Red solution...................................................................0.4mL
Preparation of Supplement Solution:  Aseptically combine com-
ponents. Mix thoroughly.
Horse Serum-Urea Solution:
Composition per 40.0mL:
Urea...............................................................................................0.2g
Horse serum, unheated.............................................................40.0mL
Preparation of Horse Serum-Urea Solution: Add urea to
40.0mL of horse serum. Mix thoroughly. Filter sterilize.
Fresh Yeast Extract Solution:
Composition :
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90
min at 15 psi pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6.6–6.8. Filter sterilize.
Penicillin Solution:
Composition per 10.0mL:
Penicillin G....................................................................... 1,000,000U
Preparation of Penicillin Solution: Add penicillin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Phenol Red Solution:
Composition per 10.0mL:
Phenol Red....................................................................................0.1g
Preparation of Phenol Red Solution: Add Phenol Red to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  Aseptically combine 140.0mL of cooled,
sterile agar base and 62.4mL of sterile supplement solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Ureaplasma urealyticum from urine. Also
used for the cultivation of other Ureaplasma species.