Esculin Broth

[所属分类:培养基配方] [发布时间:2020-7-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Esculin Broth

培养基配方

Composition per liter:
Beef heart, solids from infusion....................................................500.0g
Tryptose .....................................................................................10.0g
NaCl..............................................................................................5.0g
Agar .............................................................................................1.0g
Esculin .........................................................................................1.0g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into screw-capped tubes in 7.0mL volumes. Au-
toclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and differentiation of bacteria based on their
ability to hydrolyze esculin. Bacteria that hydrolyze esculin turn the
medium brown-black to black.