Ethyl Violet Azide Broth (EVA Broth)

[所属分类:培养基配方] [发布时间:2020-7-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ethyl Violet Azide Broth
(EVA Broth)

培养基配方
Composition per liter:
Tryptose......................................................................20.0g
Glucose........................................................................5.0g
NaCl...........................................................................5.0g
K2HPO4 ........................................................................2.7g
KH2PO4 ........................................................................2.7g
NaN3 ..........................................................................0.3g
Ethyl Violet..................................................................0.5mg
pH 6.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Use: For the isolation, cultivation, and enumeration of enterococci
from water and other specimens. Fecal enterococci turn the medium
turbid with a purple sediment on the bottom of the tube.