Eugonic HiVeg Broth

[所属分类:培养基配方] [发布时间:2020-7-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Eugonic HiVeg Broth

培养基配方
Composition per liter:
Plant hydrolysate...........................................................15.0g
Glucose .....................................................................5.0g
Papaic digest of soybean meal................................................5.0g
NaCl.........................................................................4.0g
L -Cystine...................................................................0.2g
Na2SO3 ......................................................................0.2g
Sheep blood, defibrinated..................................................50.0mL
pH 7.0 ± 2.0 at 25°C
Source: This medium, without blood, is available as a premixed pow-
der from HiMedia.
Preparation of Medium:  Add components, except blood, to dis-
tilled/deionized water and bring volume to 950.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 50°C. Aseptically add 50.0mL of sterile defibri-
nated blood. Mix thoroughly. Dispense into sterile tubes or flasks.
Use: For the cultivation and maintenance of a variety of fastidious
microorganisms, e.g., Brucella, Haemophilus, Neisseria, Pasteurella,
and Lactobacillus species.