Fastidious Anaerobe Agar (FAA)

[所属分类:培养基配方] [发布时间:2020-7-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fastidious Anaerobe Agar
(FAA)

培养基配方
Composition per liter:
Peptone......................................................................23.0g
Agar ........................................................................12.0g
NaCl..........................................................................5.0g
Glucose ......................................................................1.0g
L- Arginine ..................................................................1.0g
Sodium pyruvate...............................................................1.0g
Soluble starch................................................................1.0g
L -Cysteine·HCl·H2O ........................................................0.5g
Sodium succinate..............................................................0.5g
NaHCO3 .......................................................................0.4g
Na4P2O7 ·10H2O..............................................................0.25g
Sheep blood, defibrinated...................................................50.0mL
Hemin solution...............................................................1.0mL
Vitamin K 1  solution........................................................0.1mL
pH 7.2 ± 0.2 at 25°C
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 .................................................................1.0g
Ethanol....................................................................99.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly.
Hemin Solution:
Composition per 100.0mL:
Hemin........................................................................1.0g
NaOH (1N solution).........................................................20.0mL
Preparation of Hemin Solution:  Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water.
Preparation of Medium: Add components, except defibrinated
sheep blood, to distilled/deionized water and bring volume to
950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 50.0mL of sterile defibrinated sheep blood. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of a variety of fastidious anaerobes from clin-
ical and nonclinical specimens.