Fastidious Anaerobe Agar, Alternative Selective with Neomycin, Vancomycin, and Josamycin (FAA Alternative Selective Medium with Neomycin, Vancomycin, and Josamycin)

[所属分类:培养基配方] [发布时间:2020-7-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fastidious Anaerobe Agar, Alternative Selective with
Neomycin, Vancomycin, and Josamycin
(FAA Alternative Selective Medium
with Neomycin, Vancomycin, and Josamycin)

培养基配方
Composition per liter:
Peptone ....................................................................23.0g
Agar........................................................................12.0g
NaCl.........................................................................5.0g
Glucose......................................................................1.0g
L- Arginine..................................................................1.0g
Sodium pyruvate..............................................................1.0g
Soluble starch...............................................................1.0g
L -Cysteine·HCl·H2O........................................................0.5g
Sodium succinate.............................................................0.5g
NaHCO3 ......................................................................0.4g
Na4P2O7 ·10H2O.............................................................0.25g
Neomycin.....................................................................0.1g
Sheep blood, defibrinated..................................................50.0mL
Vancomycin solution .......................................................10.0mL
Josamycin solution.........................................................10.0mL
Hemin solution..............................................................1.0mL
Vitamin K 1  solution.......................................................0.1mL
pH 7.2 ± 0.2 at 25°C
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ................................................................1.0g
Ethanol...................................................................99.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly.
Hemin Solution:
Composition per 100.0mL:
Hemin .....................................................................1.0g
NaOH (1N solution).......................................................20.0mL
Preparation of Hemin Solution:  Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water.
Vancomycin Solution:
Composition per 10.0mL:
Vancomycin ...............................................................5.0mg
Preparation of Vancomycin Solution: Add vancomycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Josamycin Solution:
Composition per 10.0mL:
Josamycin.................................................................3.0mg
Preparation of Josamycin Solution: Add josamycin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except defibrinated
sheep blood, vancomycin solution, and josamycin solution, to distilled/
deionized water and bring volume to 930.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile defibri-
nated sheep blood, 10.0mL vancomycin solution, and 10.0mL of
josamycin solution. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the selective cultivation of Fusobacterium species from clin-
ical and nonclinical specimens.