FC Agar (Fecal Coliform Agar) (m-FC Agar) (m-Fecal Coliform Agar)

[所属分类:培养基配方] [发布时间:2020-7-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

FC Agar
(Fecal Coliform Agar)
(m-FC Agar)
(m-Fecal Coliform Agar)

培养基配方
Composition per liter:
Agar .......................................................................15.0g
Lactose.....................................................................12.5g
Tryptose ...................................................................10.0g
NaCl.........................................................................5.0g
Proteose peptone No.3........................................................5.0g
Yeast extract................................................................3.0g
Bile salts...................................................................1.5g
Aniline Blue.................................................................0.1g
Rosolic acid solution.......................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Rosolic Acid Solution:
Composition per 100.0mL:
Rosolic acid ................................................................1.0g
Preparation of Rosolic Acid Solution: Add rosolic acid to 0.2N
NaOH and bring volume to 100.0L. Mix thoroughly.
Preparation of Medium: Add 10.0mL rosolic acid solution to
950.0mL of distilled/deionized water. Mix thoroughly. Add other com-
ponents and bring volume to 1.0L with distilled/deionized water. Mix
thoroughly. Gently heat and bring to boiling with frequent mixing. Do
not autoclave. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of fecal coliform bacteria from waters and the
enumeration of coliform bacteria using the membrane filtration
method.