Fecal Coliform Agar, Modified

[所属分类:培养基配方] [发布时间:2020-7-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fecal Coliform Agar, Modified

培养基配方
Composition per liter:
Agar ....................................................................15.0g
Lactose..................................................................12.5g
Tryptose ................................................................10.0g
Proteose peptone No.3.....................................................5.0g
NaCl......................................................................5.0g
Yeast extract.............................................................3.0g
Bile salts No.3...........................................................1.5g
Aniline Blue..............................................................0.1g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium:  Add components and bring volume to
1.0L. Mix thoroughly. Gently heat and bring to boiling. Do not auto-
clave. Cool to 50°C. Adjust pH to 7.4. Pour into sterile Petri dishes in
20.0mL volumes. Allow surface of plates to dry before using.
Use: For the isolation, cultivation, and identification of stressed fecal
coliform microorganisms based on their ability to ferment lactose. Lac-
tose-fermenting bacteria turn the medium blue.