Fervidobacterium islandicum Medium

[所属分类:培养基配方] [发布时间:2020-7-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fervidobacterium islandicum Medium

培养基配方
Composition per liter:
(NH4)2SO4 ..............................................................1.3g
Yeast extract...........................................................1.0g
KH2PO4 ................................................................0.28g
MgSO4 ·7H2O...........................................................0.25g
CaCl2 ·2H2O...........................................................0.07g
FeCl3 ·6H2O...........................................................0.02g
Na2B4 ·10H2O..........................................................4.5mg
MnCl2 ·4H2O...........................................................1.8mg
Resazurin .............................................................1.0mg
ZnSO4 ·7H2O..........................................................0.22mg
CuCl2 ·2H2O .........................................................0.05mg
Na2MoO4 ·2H2O .......................................................0.03mg
VOSO4 ·2H2O..........................................................0.03mg
CoSO4 ................................................................0.01mg
Glucose solution .....................................................20.0mL
Na2S·9H2O solution...................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 20.0mL:
Glucose ...............................................................2.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 20.0mL. Mix thoroughly. Sparge with
100% N2 for 3–4 min. Autoclave for 15 min at 15 psi pressure–121°C.
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O..............................................................0.5g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Gas under 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except glucose solu-
tion and Na2S·9H2O solution, to distilled/deionized water and bring
volume to 970.0mL. Mix thoroughly. Adjust pH to 7.0. Sparge with
100% N2 . Autoclave for 15 min at 15 psi pressure–121°C. Aseptically
and anaerobically add 20.0mL of sterile glucose solution and 10.0mL
of sterile Na2S·9H2O solution. Mix thoroughly.
Use: For the cultivation of Fervidobacterium islandicum.