- 定做培养基/定制培养基
- 2020年版中国药典
- 促销/特价商品
- 院感/疾控/体外诊断/采样管
- 样品采集与处理(均质)产品
- 按标准检索培养基
- 颗粒培养基
- 标准菌株生化鉴定试剂盒
- 预灌装即用型成品培养基
- 模拟灌装用培养基
- 干燥粉末培养基
- 培养基添加剂/补充剂
- 生化反应鉴定管
- 染色液等配套产品
- 对照培养基/标准品
- 实验耗材与器具
- 生化试剂/化学试剂
- 菌种鉴定服务
F-G Broth (Feeley-Gorman Broth)
山东拓普生物工程有限公司
Shandong Tuopu Biol-Engineering Co.,Ltd
F-G Broth
(Feeley-Gorman Broth)
培养基配方
Composition per liter:
Casein, acid hydrolyzed...................................................17.5g
Beef extract...............................................................3.0g
Starch.....................................................................1.5g
L -Cysteine solution.....................................................10.0mL
Fe4(P2O7)3 solution......................................................10.0mL
pH 6.9 ± 0.05 at 25°C
L -Cysteine Solution:
Composition per 10.0mL:
L- Cysteine·HCl·H2O......................................................0.4g
Preparation of L -Cysteine Solution: Add L- cysteine·HCl·H2O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Fe4(P2O7)3 Solution:
Composition per 10.0mL:
Fe4(P2O7)3 ...............................................................0.25g
Preparation of Fe4(P2O7)3 Solution: Add Fe4(P2O7)3 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except L -cysteine solu-
tion and Fe4(P2O7)3 solution, to distilled/deionized water and bring vol-
ume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 10.0mL of L -cysteine solution. Mix thoroughly. Asepti-
cally add 10.0mL of Fe4(P2O7)3 solution. Mix thoroughly. Adjust pH to
6.9. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Legionella pneumophila.