FGTC Agar Base with Bicarbonate, Gentamicin and Amylose Azure

[所属分类:培养基配方] [发布时间:2020-7-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

FGTC Agar Base
with Bicarbonate, Gentamicin and Amylose Azure

培养基配方
Composition per liter:
Agar ......................................................................15.0g
Casein enzymatic hydrolysate ..............................................15.0g
KH2PO4 .....................................................................5.0g
Papaic digest of soybean meal...............................................5.0g
NaCl........................................................................5.0g
Galactose...................................................................1.0g
Polysorbate 80.............................................................0.75g
Thallous acetate............................................................0.5g
4-Methylumbellifery β- D -glucuronide (MUG) ...............................0.1g
NaHCO3 solution...........................................................20.0mL
Amylose azure solution ...................................................10.0mL
Gentamicin solution........................................................2.5mL
pH 7.3 ± 0.2 at 25°C
Source: This medium, without gentamicin, amylose azure, or
NaHCO3  solutions, is available as a premixed powder from HiMedia.
Gentamicin Solution:
Composition per 10.0mL:
Gentamicin.................................................................0.01g
Preparation of Gentamicin Solution: Add gentamicin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Amylose Azure Solution:
Composition per 10.0mL:
Amylose azure..............................................................3.0g
Preparation of Amylose Azure Solution: Add amylose azure to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
NaHCO3 Solution:
Composition per 20.0mL:
NaHCO3 ....................................................................2.0g
Preparation of NaHCO3 Solution: Add the NaHCO3 to distilled/
deionized water and bring volume to 20.0mL. Mix thoroughly. Filter
sterilize. Use freshly prepared solution.
Preparation of Medium: Add components, except NaHCO3 solu-
tion, amylose azure solution, and gentamicin solution, to distilled/de-
ionized water and bring volume to 970.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add 20.0mL sterile NaHCO3 solu-
tion, 10.0mL sterile amylose azure solution, and 2.5mL sterile gentam-
icin solution. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the cultivation, differentiation, and enumeration of Entero-
coccus species based on starch hydrolysis and production of fluores-
cence. Bacteria that hydrolyze starch, such as Streptococcus bovis,
appear as colonies surrounded by a clear zone. Bacteria that produce
fluorescence, such as Streptococcus bovis and Enterococcus faecium,
appear as colonies surrounded by a zone of bright bluish fluorescence
when viewed under a long-wave UV lamp. Other bacteria, such as
Enterococcus faecalis, Enterococcus avium, or Streptococcus equinus,
do not hydrolyze starch or produce fluorescence.