Fildes Enrichment Agar

[所属分类:培养基配方] [发布时间:2020-7-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fildes Enrichment Agar

培养基配方
Composition per liter:
Agar ........................................................................15.0g
Peptone.......................................................................5.0g
Beef extract..................................................................3.0g
Fildes enrichment solution..................................................50.0mL
Fildes Enrichment Solution:
Composition per 206.0mL:
Pepsin........................................................................1.0g
NaCl (0.85% solution)......................................................150.0mL
Sheep blood, defibrinated...................................................50.0mL
HCl.........................................................................6.0mL
pH 7.0–7.2 at 25°C
Source:  Fildes enrichment solution is available from BD Diagnostic
Systems.
Preparation of Fildes Enrichment Solution: Combine compo-
nents. Mix thoroughly. Incubate at 56°C for 4 hr. Bring pH to 7.0 with
20% NaOH. Adjust pH to 7.2 with HCl. Do not autoclave. Add 0.25
mL of chloroform and store at 4°C. Before use, heat to 56°C to remove
chloroform.
Preparation of Medium: Add components, except Fildes enrich-
ment solution, to distilled/deionized water and bring volume to
950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 56°C. Aseptically add
50.0mL of sterile Fildes enrichment solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Haemophilus influenzae.