Fish Peptone Broth

[所属分类:培养基配方] [发布时间:2020-7-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fish Peptone Broth

培养基配方
Composition per liter:
Maltose......................................................................5.0g
NaCl.........................................................................5.0g
Peptone......................................................................5.0g
Pancreatic digest of casein..................................................5.0g
Yeast extract................................................................5.0g
Trout tissue extract solution..............................................50.0mL
pH 7.0 ± 0.2 at 25°C
Trout Tissue Extract Solution:
Composition per liter:
Fish (brook trout)..........................................................500.0g
Pepsin......................................................................1.0g
HCl, concentrated ..........................................................15.0mL
Preparation of Trout Tissue Extract Solution: Add 1.0L of dis-
tilled/deionized water to brook trout and blend for 20–30 min. Add 1.0g
of pepsin and 15.0mL of concentrated HCl to digest the trout proteins.
Incubate for 12 hr at 45°C. Adjust pH to 7.0. Allow solids to settle. Filter
sterilize. Do not autoclave. Store at 5°C.
Preparation of Medium: Add components, except trout tissue ex-
tract solution, to distilled/deionized water and bring volume to 950.0L.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 10 psi pressure–118°C. Cool to 45°–50°C. Aseptically add 50.0mL of
sterile trout tissue extract solution. Mix thoroughly. Aseptically distrib-
ute into sterile tubes or flasks.
Use:  For the cultivation of Aeromonas salmonicida.