Flavobacterium Medium (ATCC Medium 1687)

[所属分类:培养基配方] [发布时间:2020-7-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Flavobacterium Medium
(ATCC Medium 1687)

培养基配方
Composition per liter:
Sodium glutamate................................................................4.0g
K2HPO4 ........................................................................0.65g
NaNO3 ..........................................................................0.5g
KH2PO4 ........................................................................0.19g
MgSO4 ·7H2O....................................................................0.1g
FeSO4 solution.................................................................2.0mL
pH 7.4 ± 0.2 at 25°C
FeSO4 Solution:
Composition per 10.0mL:
FeSO4 ·7H2O................................................................0.03g
Preparation of FeSO4 Solution: Add FeSO4 to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except FeSO4 solution,
to distilled/deionized water and bring volume to 998.0mL. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Aseptically add 2.0mL of sterile FeSO4 solution. Mix thoroughly. Ad-
just pH to 7.4. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Flavobacterium species.