Flexibacter Agar (LMG Medium 60)

[所属分类:培养基配方] [发布时间:2020-7-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Flexibacter Agar
(LMG Medium 60)

培养基配方
Composition per liter:
Agar ...........................................................................15.0g
Casamino acids...................................................................1.0g
Tris buffer .....................................................................1.0g
MgSO4 ·7H2O.....................................................................0.1g
KNO3 ............................................................................0.1g
CaCl2 ·2H2O.....................................................................0.1g
Sodium-β-glycerophosphate.......................................................0.1g
Thiamine........................................................................1.0mg
Vitamin B 12 ...................................................................1.0μg
Glucose solution ..............................................................10.0mL
Vitamin solution...............................................................10.0mL
Trace elements solution ........................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
Glucose .......................................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Trace Elements Solution:
Composition per liter:
H3BO3 ........................................................................2.85g
FeSO4 ·7H2O..................................................................2.49g
MnCl2 ·4H2O..................................................................1.8g
Sodium tartrate..............................................................1.77g
CaCl2 ·2H2O.................................................................40.4mg
CuCl2 ·2H2O ................................................................26.9mg
ZnCl2 .......................................................................20.8mg
Na2MoO4 ·2H2O ..............................................................25.2mg
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Vitamin Solution:
Composition per 10.0mL:
Thiamine.....................................................................10.0mg
Vitamin B 12 ................................................................10.0μg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except vitamin solu-
tion, glucose solution, and trace elements solution, to 979.0mL dis-
tilled/deionized water. Mix thoroughly. Gently heat and bring to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 10.0mL of sterile vitamin solution, 10.0mL of sterile
glucose solution, and 1.0mL of sterile trace elments solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation of Flexibacter spp.