Flexibacter canadensis Agar

[所属分类:培养基配方] [发布时间:2020-7-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Flexibacter canadensis Agar

培养基配方
Composition per 1001.0mL:
Agar .....................................................................10.0g
Casamino acids.............................................................1.0g
Tris.......................................................................1.0g
CaCl2 ·2H2O...............................................................0.1g
KNO3 ......................................................................0.1g
MgSO4 ·7H2O...............................................................0.1g
Sodium glycerophosphate....................................................0.1g
Thiamine HCl .............................................................1.0mg
Vitamin B 12 ............................................................1.0μg
Glucose solution ........................................................10.0mL
Trace elements solution SL-10 ............................................1.0mL
pH 7.5 ± 0.2 at 25°C
Glucose Solution:
Composition per 10.0mL:
Glucose .................................................................1.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Sparge with
100% N2 for 3–4 min. Autoclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution SL-10:
Composition per liter:
FeCl2 ·4H2O..............................................................1.5g
CoCl2 ·6H2O ..........................................................190.0mg
MnCl2 ·4H2O...........................................................100.0mg
ZnCl2 .................................................................70.0mg
Na2MoO4 ·2H2O ........................................................36.0mg
NiCl2 ·6H2O...........................................................24.0mg
H3BO3 ..................................................................6.0mg
CuCl2 ·2H2O ...........................................................2.0mg
HCl (25% solution).....................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl2 ·4H2O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Aseptically add 10.0mL of sterile glucose so-
lution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation of Flexibacter canadensis.