Flexibacterium Medium

[所属分类:培养基配方] [发布时间:2021-3-1] [发布人:孙绍林] [阅读次数:] [返回]

Flexibacterium Medium

培养基配方

Composition per 1060.0mL:
Yeast extract.................................................................................. 1.0g
Ca(NO3)2·4H2O ............................................................................ 0.1g
K2HPO4....................................................................................... 0.02g
Seawater, filtered ..........................................................................1.0L
Glucose solution ......................................................................50.0mL
Trace elements .........................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 50.0mL:
Glucose ......................................................................................... 1.0g
Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C.
Trace Elements:
Composition per liter:
FeSO4·7H2O............................................................................... 0.5mg
ZnSO4·7H2O .............................................................................. 0.3mg
H3BO3 ........................................................................................ 0.1mg
CoCl2·6H2O ............................................................................... 0.1mg
CuSO4·5H2O.............................................................................. 0.1mg
MnSO4·4H2O............................................................................. 0.1mg
Na2MoO4·2H2O ......................................................................... 0.1mg
Preparation of Trace Elements: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Combine components, except glucose solution. Mix thoroughly. Adjust pH to 7.2. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile glucose solution. Mix thoroughly. Aseptically distribute
into sterile tubes or bottles.
Use: For the cultivation of Flexibacter litoralis and Flexibacter marinus.